Our unit is currently addressing issues related with the mechanisms underlying the activation and the function of blood cells in inflammation by studying the role of fibrinogen (Fib) in erythrocyte and neutrophil activation and the role of neutrophil chemoattractants and blood hemodynamics in their recruitment in inflammatory conditions. Concerning this, our current and future aims are to:
For studies on the influence of Fib on erythrocyte and neutrophil biology, cellular samples will be obtained from human healthy volunteers, AMI and sepsis patients. The study of the erythrocyte NO pathway under Fib/CD47 dependence will be assessed by using pharmacological inhibitors, CD47 agonist peptides and antibodies through amperometry, spectrophotometry and fluorescence microscopy. The molecular characterization of Fib-CD47 binding will be investigated using CD47 and Fib antibodies and peptides by flow cytometry and confocal microscopy.
In AMI patients, we expect to be able to contribute for the miRNAs expression profiles with adverse cardiovascular events. Moreover, we expect to manufacture a micro-hemorheological prototype apparatus to be used in patient hospital bed side. The numerical models developed are expected to constitute useful tools for understanding the influence of biomechanical factors in the onset and progression of vascular diseases. Finally, we expect to understand how distinct chemotactic cues concert their action in neutrophil recruitment in inflammation. Altogether, we hope our studies will enable us to unravel novel potential targets and strategies for the development of therapeutics for inflammatory diseases, cardiovascular diseases as well as sepsis.
Numerical simulation of the influence of a free flowing leukocyte on the endothelial wall shear stress. Movement towards the wall disturbes the flow at longer distances.
2014/2016 Perfis da Agregação Eritrocitária e da IL-8 em sobreviventes de enfarte agudo de miocárdio. Dr. Daniel Ferreira (Hospital da Luz), Coordinator: C. Saldanha
2013/2015 Evaluation of inflammatory and adaptative immunity imbalance to improve prognosis of ischemic cardiovascular diseases, Liga dos Amigos do Hospital de Santa Marta/2012. Coordinator: Patrícia Napoleão.
2013/2015 Contribution of circulating microRNA to improve prognosis of ischemic cardiovascular diseases, Liga dos Amigos do Hospital de Santa Marta/2012. Coordinator: Patricia Napoleão
2012/2015 Mathematical and computational modeling of human physiology EXCL/Mat-NAN/0114/2012 A. Sequeira (CEMAT/IST).
2012/2015 Evaluation of inflammation profiles in septic patients – contribution to multiple cross prognostic biomarkers. Coordinator: Carlota Saldanha.
2011/2013 Functional characterization of the neutrophil in alcoholic and non-alcoholic steatohepatiti. Coordinator: Ângelo Calado.
2011/2013 Functional characterization of the neutrophil in alcoholic and non-alcoholic steatohepatitis. Coordinator: A. Calado.
2009/2013 In vivo imaging of inflammation in zebrafish: the role of chemokine system in the neutrophil and macrophage migration, Sofia de Oliveira, PhD Fellowship SFRH/BD/62674/2009, FCT. Coordinator: A. Calado.
2009/2011 Development and validation of vaso-occlusion early predictors in a a mendelian model of vascular disease, FCT. Coordinator: João Lavinha, INSA. PIC/IC/83084/2007
2009/2011 Cardiovascular risk and inflammation- contribution to the etiopathogenesis of atherosclerosis and cardiovascular disease, FCT. Coordinator: João Eurico, IMM, FML, UL. PIC/IC/82920/2007.
2007/ 2012 The relationship of circulating biomarkers of apoptosis and endothelial function with the plaque composition using VH IVUS, FCT. Coordinator: Teresa Pinheiro, Grupo de Estudos Biomédicos, Instituto Tecnológico e Nuclear (ITN). FCT project PIC/IC/82734/2007
2007/2011 Fibrinogen-dependent signalling in microvascular blood cell function – implications of inflammation and cardiovascular disease, FCT. Coordinator: Carlota Saldanha. PTDC/SAU-OSM/73449/2006
Fibrinogen-dependent signalling in microvascular blood cell function – Implications of Inflammation and Cardiovascular disease. Foundation for Science and Technology, Portugal – Project PTDC/SAU-OSM/73449/2006 (PI).
Multiscale Mathematical Modelling in Biomedicine. Foundation for Science and Technology, Portugal – Project PTDC/ MAT/ 68166/2006
Molecular Medicine – from genome to clinical practice (Team member; responsible for selection of a high-speed intravital confocal microscope). Foundation for Science and Technology, Portugal – Project REEQ/650/SAU/2005
(2002-2006) - Mathematical and Numerical Modelling in Haemodynamics – HaeModel. EU-RTN European Research Training Network - HPRN-CT 2002-00270
Mathematical and Numerical Modelling of the Human Cardiovascular System. Foundation for Science and Technology, Portugal - Project POCTI/MAT/41898/ 2001
Adélia Sequeira (Center of Mathematics and its Applications, Technical University of Lisbon, Portugal)
Victoriano Mulero (Faculty of Biology, University of Murcia, Murcia, Espanha)
Stephen Andrew Renshaw (MRC Centre for Development and Biomedical Genetics, University of Sheffield, Sheffield, Reino Unido)
Nadia Antonova, Institute of Mechanics and Biomechanics of the Bulgarian Academy of Sciences, Bulgary
Caterina Faggio, Departamento de Ciência Biológica e Ambiental, Universidade de Messina, Sicily, Italy, ERASMUS Program
Sarka Necasova, Institute of Mathematics of the Academy of Sciences of the Czech Republic, Czech Republic
Narla Mohandas, New York Blood Center, USA
Hisayuki Nomiyama (Graduate School of Medical Sciences - Department of Molecular Enzymology, Kumamoto University, Kumamoto, Japão)
Naoki Osada (National Institute of Biomedical Innovation, Osaka, Japão)
Rui L. Reis (3Bs Research Group, Department of Polymer Engineering, University of Minho, Guimarães, Portugal)
Luís L. Cardoso (Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal)
Manuela Rodeia (Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Lisboa, Portugal)
João Garcia da Fonseca, Nuno Reis, Ricardo Cabeça (Biosurfit SA, Lisboa, Portugal)
Geert Schmid-Schönbein (Microcirculation Lab/ Departament of Bioengineering, University of California, San Diego, EUA)
Gerard Nash (Center of Cardiovascular Sciences, University of Birmighan, Reino Unido)
Hermona Soreq (Faculty of Science, Hebrew University, Jerusalem, Israel)
Felix M. Gõni (Biophysics Unit, University of Basque Country, Espanha)